Fig 1: Upregulation of H3K4me3 increases iron exporter Fpn1 and exhibits neuroprotection.a, b CPI-455 treatment (24 h) increased the protein level of H3K4me3 and Fpn1 in SH-SY5Y cells. c MTT assay showed CPI-455 showed neuroprotection against the toxicity of 6-OHDA in SH-SY5Y cells. d–g SH-SY5Y cells were infected by AAV-KDM5C-silencing virus, and KDM5C was significantly decreased at the mRNA level as reflected by RT-PCR d and at the protein level by Western blot e, f. In addition, Fpn1 and H3K4me3 were increased in AAV-KDM5C-silencing virus infected cells e, f. Moreover, g silencing KDM5C showed a slight neuroprotection against 6-OHDA-induced PD model in SH-SY5Y cells. *P < 0.05; **P < 0.01; ***P < 0.001. Data are presented as mean ± SEM. n = 3–4 for each group in Western blot and n = 5 for each group in MTT assay.